The Varian Unity 400 is a multinuclear FT-NMR instrument. It's Oxford superconducting magnet at 400 MHz (9.4 Tesla) is an integral part of an NMR spectrometer. It has four probes and the following capabilities.
Deuterium lock and unlocked mode of operation. Homonuclear and heteronuclear proton decoupling. Variable temperature range varies with the probes. A variety of software is available for both one and two dimensional NMR experiments. Data may be stored and accessed through FTP. This system has a UNIX operating system with Varian VNMR software 6.3C, running on a SUN Ultra 60 computer. The computer, software, and electronics were upgraded in 1998 and the computer in 2007, a new probe was added in 1999. See a cut open view of a super conducting magnet.
Unity 400 (Room 130) - There are several probes associated with this instrument:
See the reservation protocol , form to submit liquid NMR sample, Unity 400 shim values, and the reset procedure after a power failure.
The inverse or indirect detection probe has three observe/excation channels. The outer (X Channel/coil) is normally used to excite a nuclear spin system in the X-band, the result being observed in the 1H spin system through the inner coil (Observe Channel). The inner coil is double tuned to allow simultaneous observation of 2H to facilitate a field frequency lock. The normal frequencies are:
Go to some operational procedures for obtaining spectra with the Unity 400 and specific procedure for printing spectra selecting the printer/plotter and creating experiments. Or step-by-step NMR procedures for the Unity 400 procedures for obtaining proton or carbon spectra are also described. A seperate protocol is described for One Pulse and 2-D Cosy experiments, Proton Homonuclear Decoupling Tests, meaning of the Functions Keys, or VNMR parameters and consequence.
