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New Mexico State University
College of Arts and Sciences
Department of Chemistry and Biochemistry

The Varian Unity Plus 500-MHz Nuclear Magnetic Resonance (NMR) spectrometer is a narrow-bore system equipped with a Varian Unity Plus 500 MHz Magnetconsole. The instrument is used primarily to determining the three-dimensional structures of small proteins in solution by multidimensional NMR. The Oxford magnet is supported on a pneumatic anti-vibration table (not shown). The console is equipped with three radio frequency channels and a Z-axis gradient amplifier. Solutions probes include a H1{C13}{N15} triple resonance probe. This is a proton observation probe with both Carbon and Nitrogen decoupling coils. The console is controlled by a Sun Ultra 10 computer connected to the network.

The 500 MHz NMR Spectrometer is equipped with the following:

  1. Narrow bore Oxford magnet
  2. Highband 1st and 2nd Channels with frequency ranges of 12 - 609.5 MHz and 499.5 - 500.5 MHz
  3. Broadband 3rd Channel with frequency ranges of 12 - 309.5 MHz (Both with a resolution of 0.1 Hz)
  4. 50W (pulsed) high band linear amplifiers for the 1H/19F channels
  5. 300 W (pulsed) broadband linear amplifiers (12 - 200 MHz, and 150 W for 200 - 245 MHz)
  6. Two additional 300W (pulsed) broadband linear amplifiers (300 W at 12 - 200 MHz, 150 W for 200 - 245 MHz) on the 3rd channels
  7. A Performa II Pulsed-Field Gradient modules which provide > 30 gauss/cm
  8. Deuterium decoupling accessories
  9. VT control
  10. Ultrashims II
  11. Waveform generators on each channel (for shaped and comp. pulses)
  12. High sensitivity, low noise GaAs FET preamplifiers for 1H/19F
  13. A 2H lock with computer-controlled lock gating (for deuterium decoupling experiments)
  14. A 16-bit ADCs, 500 kHz spectral widths and full quadrature with simultaneous sampling and oversampling

500 MHz System

 

 

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