Principle -- Computer controlled ratio mode for fluorescence spectrophotometer with measurement modes for fluorescence, phosphorescence, chemiluminescence and bioluminescence.
Specifications: Monochromators
Excitation: Czerny-Turner, f3.6, 0.125 m focal length, Emission: Czerny-Turner, f3.6, 0.125 m focal length,
Gratings
Excitation: 30 x 35 mm, 1200 l/mm, blaze at 370 nm, Emission: 30 x 35 mm, 1200 l/mm, blaze at 440 nm
All reflective optical system with quartz over-coated optics, Schwarzschild source optics for increased energy throughput and precise imaging and focusing, 80 data points per second maximum measurement rate in fluorescence mode, non- measurement phase stepping wavelength drive, room light immunity in fluorescence mode, centrally controlled by PC with Windows 2000 operating systems. Source Xenon pulse lamp with exceptionally long lifetime, pulsed at 80 Hz. Pulse width at half peak height ~ 2 µs, peak power equivalent to 75 kW. Wavelength range (nm) Mechanical Excitation: 190 to 1100 nm. Zero order selectable. Emission: 190 to 1100 nm. Zero order selectable. Operational Excitation: 200 to 900 nm with standard PM tube. Zero order selectable. Emission: 200 to 900 nm with standard PM tube. Zero order selectable. Wavelength accuracy (nm) ± 1.5 nm Wavelength reproducibility (nm) ± 0.2 nm
Detectors High performance R928 photomultiplier detector and separate R928 PMT for reference signal.
Sensitivity (Raman Band of Water) >500:1 RMS, 500 nm excitation, excitation and emission slits 10 nm, 1 sec Signal Averaging time. >750:1 RMS, 350 nm excitation, excitation and emission slits 10 nm, 1 sec Signal Averaging time.
Minimum sample volume 0.5 mL (using standard 10-mm fluorescence cell) Filters are selectable, open, closed, auto and one of the following:
The temperature controller is an electrothermal device which heats and cools samples in the sample compartment by heat exchange devices (Pelters) positioned around the cell. It uses a built in water reservoir to prevent the cell compartment from reaching high temperatures. Temperature is set through the Cary software. A side view of the apparatus is show on the right it sets on the floor under the table holding the spectrophotometer. A similar device is used for the Cary UV/VIS spectrophotometer.
Temperature maybe adjusted in the range from -10 oC to 100 oC.
